Joined: 15 Aug 2006
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Posted: Fri Aug 07, 2009 5:59 pm Post subject: AnaSpec Introduces New SensoLyte® 520 Thiol Quantitation Kit
San Jose, CA – August 7, 2009
Thiol compounds, such as glutathione (GSH), cysteine, and homocysteine, are a natural reservoir of the reductive capacity of a cell. They function as components of the intracellular and extracellular redox buffer and play important roles in a variety of biological processes, such as enzyme catalysis, redox-signaling protein folding, and free radical scavenging.1-3
AnaSpec has announced the release of the SensoLyte® 520 Thiol Quantitation Kit (patent pending), an ultra-sensitive, robust and easy-to-use fluorescent assay for measurement of total thiol level. This kit contains a novel fluorogenic reagent which upon reaction with thiol releases fluorescence. Fluorescence signal can be detected at the common green excitation/emission wavelengths of 490 nm/520 nm. The assay has a linear range of 0.04-160 mM thiol. Common contaminants, e.g. DMSO, and Triton X-100, are well tolerated in the assay. Additionally, autofluorescence interference from cellular components or from test compounds is minimal at the emission wavelength of 520 nm.
AnaSpec also offers another fluorimetric kit, the SensoLyte® ABD-F Thiol Quantitation Assay Kit; as well as a colorimetric kit, the SensoLyte® Thiol Quantitation Assay Kit.
1. Dickinson, DA. et al. Biochem. Pharmacol. 64, 1019 (2002).
2. Winterbourn, CC. et al. Free Radical Biol. Med. 45, 549 (2008).
3. Franco, R. et al. Arch. Physiol. Biochem. 113, 234 (2007).
AnaSpec is a leading provider of integrated proteomics solutions to the world’s largest biotech, pharmaceutical, and academic research institutions. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents, and antibodies.
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