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AnaSpec Introduces Calcein AM for Live Cell Staining

 
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anaspec



Joined: 15 Aug 2006
Posts: 238
Location: 34801 Campus Drive, Fremont, CA 94555

PostPosted: Tue Aug 25, 2009 12:23 pm    Post subject: AnaSpec Introduces Calcein AM for Live Cell Staining Reply with quote

San Jose, CA – August 25, 2009

Calcein AM (Calcein acetoxymethyl ester), a non-fluorescent, cell permeable compound, converts to the strongly green fluorescent calcein when hydrolyzed by intracellular esterases in live cells.1-2 It is widely used for determining cell viability since the fluorescence intensity of calcein is proportional to the amount of live cells. Calcein is a photostable reagent and is well retained in the cytoplasm of live cells. Calcein is not influenced by intracellular pH. Its bright green fluorescence can be monitored at Ex/Em=494nm /520 nm.

Amongst other reagents, including BCECF, AM and Carboxy-fluorescein diacetate, Calcein AM is the most suitable indicator for staining viable cells due to its low cytotoxicity property. Calcein AM does not affect cell proliferation or viability.2-3 Calcein AM is used in cell invasion, adhesion, migration and other cell-based assays.

AnaSpec provides Calcein AM at two purity grades, >90% or >95%, as determined by HPLC. AnaSpec also offers the SensoLyte® Calcein Cell Viability Assay Kit - a rapid and sensitive assay to detect viable cells. This kit provides ample reagents (dye and assay buffer) for 1000 assays in a 96-well plate format.

References
1. Braut-Boucher, F. et al. J. Immunol. Methods 178, 41 (1995).
2. Akeson, AL. et al. J. Immunol. Methods 163, 181 (1993).
3. Weston , SA. et al. J. Immunol. Methods 133,

About AnaSpec

AnaSpec is a leading provider of integrated proteomics solutions to the world’s largest biotech, pharmaceutical, and academic research institutions. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents, and antibodies.

For more information visit www.anaspec.com

###
_________________
Ping Yang
Marketing Speialist
34801 Campus Drive
Fremont, CA 94555
1-800-452-5530 (Toll free)
1-510-791-9560 (Tel)
1-510-791-9572 (Fax)
ping@anaspec.com
www.anaspec.com
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