Joined: 15 Aug 2006
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Posted: Wed Jun 20, 2007 6:46 pm Post subject: AnaSpec Introduces New SensoLyte™ Beta-Secretase Assay Kit
June 18, 2007 – San Jose, CA
Leveraging one of the world’s largest collections of amyloid peptides, AnaSpec has introduced its latest development in novel solutions for amyloid-related research - the new SensoLyte™ 520 Beta-Secretase Assay Kit.
Beta-secretase catalyzes a key step in the production of beta-amyloid peptides seen accumulated in senile plagues of Alzheimer’s disease (AD) brains. In order to facilitate high throughput screening of AD drug candidates, we have developed a new SensoLyte™ 520 beta-secretase assay kit using a fluorescence resonance energy transfer (FRET) peptide, HiLyte Fluor™ 488-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(QXL™ 520)-OH (see Figure 1). The sequence of this FRET peptide is derived from the beta-secretase cleavage site of beta-amyloid precursor protein(APP) with Swedish mutation.6 This mutation enhances the susceptibility of APP to beta-secretase and results in an early onset of AD.
This assay has good sensitivity (0.03 mU/ml) with a signal-to-background ratio >10 after a 30-minute incubation. This homogeneous assay can be used to continuously monitor product formation. Validated with known inhibitors, the IC50 value for one of the inhibitors determined with SensoLyte™ 520 beta-secretase assay kit was consistent with published data.
AnaSpec, Inc. is a leading provider of integrated proteomics solutions to pharmaceutical, biotech, and academic research institutions throughout the world. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents (dyes, assay kits, & antibodies), and combinatorial chemistry. Established in 1993, AnaSpec's ISO9001:2000 certified headquarters and manufacturing facilities are located in San Jose, CA.
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