Joined: 15 Aug 2006
Location: 34801 Campus Drive, Fremont, CA 94555
Posted: Tue Jan 22, 2008 4:19 pm Post subject: AnaSpec Introduces New Line of HABA-Biotin Quantitation Kits
January 22, 2008 – San Jose, CA
AnaSpec has introduced a new line of HABA Biotin Quantitation Kits. These kits provide a convenient method for estimating the molar ratio of biotin to protein on biotinylated conjugates. They can also be used to quantitate the biotin concentration in a solution.
Available in two sizes:
120 Assays 72096-120 (120 assays in a 96-well format or 25 assays in a cuvette format)
500 Assays 72096-500 (500 assays in a 96-well format or 100 assays in a cuvette format)
Biotin and avidin (or streptavidin) bind non-covalently with a higher binding affinity than most antigen-antibody interactions. This very tight binding makes labeling proteins with biotin a useful tool for applications such as affinity chromatography and immunoanalytical assays.
The assay utilizes the observation that HABA (4’-hydroxyazobenzene-2-carboxylic acid) shows dramatic spectral changes when it binds to avidin. Free HABA has an absorption peak at 348 nm, while the HABA/avidin complex has strong absorption at 500 nm. Since the affinity between HABA and avidin is relatively weak (Kd=5.8 x 10-6 M) compared to the affinity between biotin and avidin (Kd=1 x 10-15 M), biotin can easily replace HABA from the HABA/avidin complex, resulting in a decrease of absorption at 500 nm. Avidin and HABA at an optimal ratio are included in the assay kit. The kit has a linear range of 2 to 16 M.
AnaSpec, Inc. is a leading provider of integrated proteomics solutions to pharmaceutical, biotech, and academic research institutions throughout the world. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents (dyes, assay kits, & antibodies), and combinatorial chemistry.
For more information, visit www.anaspec.com _________________ Ping Yang
34801 Campus Drive
Fremont, CA 94555
1-800-452-5530 (Toll free)
You cannot post new topics in this forum You cannot reply to topics in this forum You cannot edit your posts in this forum You cannot delete your posts in this forum You cannot vote in polls in this forum